The aim of a new study published in the International Journal of Gynecology and Obstetrics was to determine the diagnostic value of vaginal discharge concentrations of β human chorionic gonadotropin (β-hCG), creatinine, and urea to identify premature rupture of membranes (PROM). Here, an observational cross-sectional study was conducted between 12 June, 2014, and 11 June, 2015, among 120 women who were admitted to an Iranian hospital at 28-41 weeks of pregnancy. Subsequently, 60 women who had positive pooling and nitrazine test results were assigned to the PROM group, while the rest having negative test results were assigned to the control group. The results revealed that the mean concentrations of β-hCG, urea, and creatinine were all higher in the PROM group than in the control group. Meanwhile, the diagnostic accuracies as defined by area under the curve for creatinine, urea, and β-hCG were 0.891, 0.895, and 0.908, respectively. In addition, the corresponding optimal cutoff values were 20.33 μmol/L, 0.94 mmol/L, and 39.5 IU/L, respectively. Thus, it was stated that the vaginal discharge concentrations of all three markers exhibited favorable predictive value for the diagnosis of PROM; however, β-hCG showed greater diagnostic accuracy than either urea or creatinine.