The purpose of a new study published in the Journal of Photochemistry and Photobiology was development and control of an efficient photodynamic inactivation of onychomycosis causative pathogens. This study evaluated the usage of time-resolved 2D singlet oxygen luminescence detection in combination with 2D fluorescence scanning as a tool to understand the behavior of the photosensitizer when applied to fungi on Petri dishes. Two different photosensitizer, a cationic porphyrin, and cationic corrole and two fungi strains, the dermatophyte Trichophyton rubrum and the mold Scopulariopsis brevicaulis, were investigated. The findings showed differences in the diffusion of both photosensitizer. Despite the singlet oxygen luminescence was quenched with increasing growth of fungi, the kinetics of singlet oxygen luminescence could be detected on Petri dishes for both photosensitizers and both fungi strains for up to seven days.¹

Reference

1. Bornhütter T, Shamali N, Saltsman I et al. Singlet oxygen luminescence kinetics under PDI relevant conditions of pathogenic dermatophytes and molds. Journal of Photochemistry and Photobiology B: Biology. 2018;178:606-613. doi:10.1016/j.jphotobiol.2017.12.015.