Aim- A study investigated if the anti-inflammatory effect of EA 575® is due to an impact on the NFκB pathway.

Study Design and Method-

• Immunofluorescence was utilized to observe NFκB nuclear translocation in J774.2 and HEK293 cells.
• A luciferase-based reporter was used to monitor NFκB transcriptional activity in HEK293 cells.
• Western blot analysis evaluated the Phosphorylation of RelA and its inhibitor IκB.
• Protein fragment complementation was used to exhibit changes in the stability of the NFκB:IκB complex. 

Results-

Conclusion: EA 575® can regulate the NFκB pathway, likely by shifting specificity of IKK from IκBα to RelA, thus causing increased stability of NFκB:IκBα complex and decreased RelA translocation into the nucleus.

Source: Inflammopharmacology. 2019 Apr;27(2):339-347. doi: 10.1007/s10787-018-0494-9.