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EM and Other Stains - When and Where?

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Dr Manoj Jain, Lucknow    28 November 2019

  • The combination of light microscopy (LM), immunofluorescence (IF) microscopy and transmission electron microscopy (TEM) along with clinical details contributes to a confident and accurate diagnosis in majority of renal biopsies.
  • All renal biopsies are examined with light microscope multiple by serial sections cut at 3 micron and stained with routine. H&E, periodic acid-Schiff’s (PAS), PASM (Silver) and masson trichrome (MT) stain.
  • IF microscopy for IgG, IgM, IgA, C3, C1q and kappa lambda light chain is done in all renal biopsies. C4d is done transplant biopsies.
  • TEM is used to obtain structural and compositional information which cannot be acquired using LM and IF or with other alternative technique e.g., podocytopathy, Alport’s syndrome, deposition diseases, etc.
  • Additional immunohistochemistry (IHC)/IF used to analyze renal native and transplant biopsies.

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