A new article published in Molecules reported on a first evaluation of the photoprotective effect of fish bone bioactive peptides (FBBP) preparation isolated from silver carp (Hypophthalmichthysmolitrix) discarded tissue, using in vitro experimental models of skin cells exposed to ultraviolet B (UVB) irradiation and stressing agents. 

For this experiment, FBBP preparation was obtained by papain treatment of minced bones and centrifugal ultrafiltration, and the molecular weight (MW) distribution was characterized by size exclusion and reversed-phase high performance liquid chromatography (RP-HPLC). 

Assessment of the effect of FBBP pretreatment in UVB-irradiated L929 fibroblasts and HaCaT keratinocytes revealed their cytoprotective activity. Their capacity to efficiently reduce reactive oxygen species (ROS) production and lipid peroxidation varied in a dose-dependent manner, and was greater in fibroblasts. FBBP pretreatment of THP-1-derived inflamed macrophages rendered reduction in the proinflammatory cytokines secretion, particularly, of tumor necrosis factor alpha (TNF-α). UVB-irradiated Mel-Juso cells showed lowered melanin production and tyrosinase activity, and were directly proportional to FBBP concentrations. Furthermore, FBBP fractions with high radical scavenging activity were separated by ion exchange chromatography –two collagenic sequences were identified. 

Thus, bioactive peptides derived from discarded H. molitrix bones might have an important impact on skin cells metabolism as dataconfirm their ability to control the antioxidant, anti-inflammatory and pigmentation processes—developed during UV irradiation of skin cells. 

Source: Molecules. 2021 May; 26(9): 2691.doi: 10.3390/molecules26092691