A systematic review and meta-analysis assessed the impact of exercise during pregnancy on gestational diabetes mellitus (GDM). PubMed, EMbase, Web of science, EBSCO, and Cochrane library databases were systematically searched and randomized controlled trials (RCTs) evaluating the impact of exercise during pregnancy on GDM were included. Six RCTs involving 2164 patients were included in the meta-analysis. Exercise intervention was linked with significantly decreased incidence of GDM in comparison with control intervention; however, it had no effect on gestational age at birth, the number of preterm birth, glucose 2-h post-OGTT, birth weight, and Apgar score <7. Therefore, compared to control intervention, exercise intervention during pregnancy appeared to significantly decrease the risk of GDM. The report is published in the Journal of Maternal-Fetal and Neonatal Medicine.