Virulence and antimicrobial resistance pattern in diarrhoeagenic Escherichia coli.


eMediNexus    24 July 2018

A new study published in the Journal of Health, Population, and Nutrition aimed to determine virulence pattern and antibiotic resistance among the circulating diarreagenic Escherichia coli (DEC) strains, from a tertiary care center in south of India. Here, diarrheal stool specimens were obtained from 120 children below 5 years of age and 100 adults above 18 years, which were subjected to culture and isolation of pathogens. Conventional PCR was performed to detect 10 virulence and 27 antimicrobial resistance (AMR) genes among E. coli isolates. The results revealed DEC infection in 37.5% children and 18% adults, among which atypical emergence of atypical enteropathogenic Escherichia coli (EPEC) was the most common, followed by enteroinvasive Escherichia coli (EIEC), typical EPEC, and shiga toxin-producing Escherichia coli (STEC). While necrotoxic Escherichia coli (NTEC) and cell-detaching Escherichia coli (CDEC) were not detected. DEC co-infection was found in 6.6% children and 1% adults and sole-hybrid DEC infection was detected in 6.6% children. Meanwhile, the distribution of sulphonamide resistance genes and class 1 integron (int1) genes was higher in DEC strains isolated from children and adults. From the findings, it was inferred that atypical EPEC was a primary etiological agent of diarrhea in children and adults among the DEC pathotypes. Detection of high numbers of AMR genes and class 1 integron genes indicate the importance of mobile genetic elements in spreading of multidrug resistance genes among these strains.

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