The goal of a recent study published in the Italian Journal of Pediatrics was to evaluate the effect of a probiotic mixture (PM), including Bifidobacteria breve, infantis, and longum on childhood functional constipation (FC). Here, 55 consecutive children suffering from FC were randomly assigned into two groups – group A received a daily oral combination of polyethylene glycol (PEG) plus PM, while group B received oral PEG only. Physical and clinical data were collected from each patient at weeks 1,2, and 8. The results showed that after 1 month, children who experienced improvement in the PEG and in the PEG + PM group were 88 and 81.8%, respectively. In addition, 1 month after the end of the study treatment, a positive trend towards a higher rate of clinical remission was observed among children treated with PEG + PM. The findings suggested that PEG and PEG + PM are equally effective and safe in the treatment of children with chronic constipation. It was stated that further studies are necessary to ascertain whether adding Bifidobacteria strains to conventional therapy may lead to a better long-term outcomes.