Urinary tract infection (UTI) is one of the most common medical complications seen in pregnant women, and the most common causative organism is Escherichia coli. A study published in the International Journal of Health Sciences and Research estimated the incidence of bacterial pathogens causing UTI among pregnant women and also sought to detect the antimicrobial sensitivity patterns and the uropathogenic markers. In all, 300 samples were investigated from pregnant women aged 18-35 years. Women with varying gravida and irrespective of all three trimesters were included over a period of one year. The identified pathogens were screened for pathogenic factors including hemolysin production, mannose resistant and mannose sensitive hemagglutination (MRHA, MSHA), cell surface hydrophobicity and serum resistance by recommended methods. Of the 300 samples collected, 117 (39%) samples were sterile and rest 183 samples (61.0%) showed bacterial growth. The prevalence of UTI was found to be 61% and the highest predominant uropathogen was E.coli (74.3%). Since E. coli accounted for majority of isolated organisms, those were subjected to evaluation for various pathogenic markers. The most common factor detected was serum resistance in 115 (84.5%) cases while cell surface hydrophobicity was found to be the least common virulence factor.