A new study published in Medical Science Monitor analyzed the differences in vaginal microecological factors and genital tract infections among pregnant women of different ages. Here, 751 pregnant women were selected from the West China Second University Hospital, China, from January 2015 to April 2017. The vaginal microecological factors of these women were assessed, which included vaginal cleanliness, lactobacillus number, bacterial density, flora diversity, dominant bacteria, vaginal pH, clue cells, Candida species, and Trichomonas vaginalis. The results showed no significant difference in bacterial density, flora diversity, vaginal cleanliness or lactobacillus number among pregnant women of different age groups. On the other hand, among the 32.62% of pregnant women who had genital tract infections, the incidence of bacterial vaginosis, Candida albicans infection, non-albicans Candida infection, and T. vaginalis infections were 20.91%, 14.91%, 4.26%, and 1.73%, respectively. While the amalgamative incidence of bacterial vaginosis was 9.19%. The incidence of non-albicans Candida infection in the optimum reproductive age group was higher than in the older age group. Whereas, the incidence of T. vaginalis infection in the younger age group was higher than those in the optimum reproductive age group and the older age group. From the findings, it was inferred that the microecological status of pregnant women was similar to that of normal women. The most frequent genital tract infection was bacterial vaginosis. While bacterial vaginosis was found to be amalgamative with vulvovaginal candidiasis and T. vaginalis infections, there was no significant difference in vaginal microecological observations among pregnant women in different age groups except that the non-albicans Candida infection incidence in the optimum reproductive age group and the T. vaginalis infection incidence in the younger age group was higher than in the other groups.